T4 RNA Ligase, Promega

Supplier: Promega

M1051

PAM1051EA 172.03 USD

PAM1051

T4 RNA Ligase, Promega

Nucleic Acid Reagents Cloning Kits and Ligation

T4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5'-phosphoryl termini of single-stranded RNA or DNA.

Joins ssRNA or ssDNA onto the 5´-Phosphoryl End of ssRNA or ssDNA
Adds [5´-32P] nucleoside 3´,5´-bis (phosphate) onto single-stranded RNA
Can be inactivated by heating at 65°C for 15 minutes
Supplied at a concentration of 10 u/microl
For ligation, Labeling and cloning

T4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5'-phosphoryl termini of single-stranded RNA or DNA. The enzyme, purified from recombinant E. coli CA4 (RNase I-deficient), has an apparent molecular weight of 43.5kDa. T4 RNA Ligase also catalyzes the addition of [5'-32P] nucleoside 3',5'-bis (phosphate) onto single-stranded RNA.

Caution: Storage conditions –30...–10 °C

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