innuSCRIPT One Step RT-PCR SyGreen Kit, Analytik Jena

Supplier: Analytik Jena
innuSCRIPT
845-RT-6000100 845-RT-6000200 845-RT-6000200
10755-360EA 870.57 USD
10755-360 CA10755-360 CA10755-358
innuSCRIPT One Step RT-PCR SyGreen Kit, Analytik Jena
Nucleic Acid Reagents qPCR/RT-qPCR Enzymes and Kits
The innuSCRIPT One Step RT-PCR SyGreen Kit was developed for fast, highly reproducible reverse transcription and subsequent real-time PCR, and has been validated on the most common real-time PCR instruments. The innuSCRIPT One Step RT-PCR SyGreen Kit includes a dye that intercalates with double-stranded DNA without inhibiting the PCR reaction as many traditional PCR fluorescent dyes do.

  • Reverse transcription and real-time PCR in a single step
  • Real-time PCR using an intercalating dye
  • Simple, well-established handling and superior reproducibility
  • Compatible with most real-time PCR thermocyclers
  • Highly specific, efficient reverse transcription and subsequent real-time amplification

The innuSCRIPT One Step RT-PCR SyGreen Kit was developed for fast, highly reproducible reverse transcription and subsequent real-time PCR, and has been validated on the most common real-time PCR instruments

The concentration of MgCl2 in the master mix is already ideal, eliminating the need to add any more.

The innuSCRIPT One Step RT-PCR SyGreen Kit includes a dye that intercalates with double-stranded DNA without inhibiting the PCR reaction as many traditional PCR fluorescent dyes do. The 2x concentrated master mix also contains a thermostable reverse transcriptase (50°C to 55°C) and an effective RNase inhibitor. The composition of the innuSCRIPT One Step RT-PCR SyGreen Kit has been optimized to yield an excellent slope at optimum PCR efficiency. Users now only need to add the RNA template and primers to the reaction, followed by enough water (suitable for real-time PCR) to produce the final volume.
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