The innuSPEED Bacteria/Fungi RNA Kit has been designed for rapid homogenization and includes an efficient process for digesting the cell walls of fungal spores and gram+ bacteria in specially developed lysis tubes.
- Extraction of total cellular RNA from fungal spores and gram+ bacteria
- No DNase I digestion: filter membrane for fast, selective removal of genomic DNA
- Integrated inactivation of endogenous and exogenous RNases
- Improved extraction without the use of toxic β-mercaptoethanol
The innuSPEED Bacteria/Fungi RNA Kit has been designed for rapid homogenization and includes an efficient process for digesting the cell walls of fungal spores and gram+ bacteria in specially developed lysis tubes; the process utilizes the Analytik Jena SpeedMill PLUS (or other homogenizer). The starting sample is then denatured. The unique lysis buffer deactivates endogenous and exogenous RNases. Once the genomic DNA has been selectively removed, the RNA is bound to the surface of a spin filter membrane, washed, and then finally eluted from the membrane. The isolated RNA is immediately available for downstream applications.
Specifications
Starting material: Gram+ bacteria (max. 1 × 10^9); Fungal spores
Column binding capacity: > 30µg RNA
Average purity: 1.7 – 2.0
Homogenization and lysis: approx. 40 minutes
Purification: approx. 10 minutes
Average yield: Up to 30µg, depending on the type and quantity of starting material