The innuPREP Plasmid Mini Kit allows researchers to isolate plasmid DNA from bacterial lysates quickly and easily.
- A combination kit for extracting high-copy and low-copy plasmid DNA, P1 constructs, etc.
- Final yields of up to 40µg of highly pure plasmid DNA
- Starting volumes of the bacterial suspensions to be used covers a large range (0.5-10ml)
- The eluted pDNA can be used immediately prior to sequencing
The innuPREP Plasmid Mini Kit allows researchers to isolate plasmid DNA from bacterial lysates quickly and easily. The method combines alkaline lysis with the process of binding plasmid DNA to a filter membrane once the chromosomal DNA and bacterial proteins have been precipitated. The bound plasmid DNA is then washed and eluted by adding a low salt buffer. The resulting isolated plasmid DNA can then be used immediately in a variety of additional downstream applications. The new method allows scientists to isolate plasmid DNA from 0.5-10ml of starting material. The use of a novel binding membrane makes it possible to isolate up to 40µg of pDNA.
Specifications
Starting material: Bacterial suspensions; Isolation of high-copy plasmids: 0.5-5ml; Isolation of low-copy plasmid DNA, P1 constructions, etc: > 5-10ml
Column binding capacity: approx. 40µg pDNA
Average purity: 1.8 – 2.0
Extraction time: Approx. 16 minutes
Typical yield from 2 ml starting material (high-copy plasmid): 6-20µg
