The innuPREP Bacteria DNA Kit has been optimized specifically for isolating bacterial DNA from cell pellets after culturing, making it possible to process both gram+ and gram– bacteria.
- Bacterial DNA extraction from gram+ and gram– cell cultures
- Patented DC technology for short lysis times and efficient binding of bacterial DNA
- DNA isolation from up to 1 × 109 bacterial cells
- Certified for in-vitro diagnostic applications (CE-IVD)
The innuPREP Bacteria DNA Kit has been optimized specifically for isolating bacterial DNA from cell pellets after culturing, making it possible to process both gram+ and gram– bacteria. The extraction process combines an initial lysis step using lysozyme and a subsequent proteolytic digestion step with a highly efficient process for binding bacterial DNA to the surface of a spin filter membrane. The DNA is then washed and desorbed from the surface of the filter. Extraction is based on spin filter columns and, in addition to being simple to perform, makes it possible to bind more than 50 µg of bacterial DNA. CE-IVD marking also makes the innuPREP Bacteria DNA Kit suitable for in-vitro diagnostics.
Specifications
Starting material: Gram+ and gram– bacterial cell pellets after culturing - Up to 1 × 10^9 cells
Column binding capacity: > 50 µg DNA
Average purity: 1.7 – 2.0
Extraction time: Approx. 45 minutes
Average yield: Depends on the type and starting quantity and/or cell count of the bacteria; up to 35 μg
