The innuCONVERT Bisulfite All-In-One Kit allows researchers to extract DNA from a variety of starting materials, and subsequently completely convert non-methylated cytosine to uracil in only a few hours.
- DNA extraction for various starting materials
- Includes a module for completely converting non-methylated cytosine to uracil in minutes
- Liquid reagents are stable and can be stored
- Denaturation and conversion combined in a single work sequence
- Overall optimized process is complete in under 5 hours
The innuCONVERT Bisulfite All-In-One Kit allows researchers to extract DNA from a variety of starting materials, and subsequently completely convert non-methylated cytosine to uracil in only a few hours. A specially optimized universal buffer can be used for processing different lysis protocols, while DNA sample/lysate denaturation and bisulfite treatment take place in a single reaction vessel. This is followed by on-column purification and desulfonation of the converted DNA. The pure nucleic acid obtained can then be easily used for later downstream applications (such as PCR and sequencing).