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Angiotensin I Converting Enzyme (ACE1) Inhibitor Screening Kit, BioVision Inc.

Supplier: BioVision
K228-100
Industry's first fluorometric kit to screen/study/characterize potential inhibitors of ACE1.

  • Simple one-step reaction
  • Takes only one to two hours
  • Non-radiometric fluorescent screening process
  • HTP adaptable

Angiotensin I converting enzyme (ACE1, EC: 3.4.15.1), a dipeptidyl carboxypeptidase, is part of the renin-angiotensin system (RAS) that controls regulation of blood pressure by cleaving the C-terminal dipeptides of angiotensin I and bradykinin. It is found on the luminal surface of vascular endothelial cells, especially in pulmonary tissues. In addition, elevated levels of ACE1 are found in sarcoidosis, leprosy, hyperthyroidism, acute hepatitis, primary biliary cirrhosis, diabetes mellitus, multiple myeloma, osteoarthritis, amyloidosis, Gaucher disease, pneumoconiosis, histoplasmosis and miliary tuberculosis. BioVision’s ACE1 Inhibitor Screening Kit can be used to screen for potent inhibitors of ACE1 activity to regulate hypertension. It utilizes the ability of an active ACE1 to cleave a synthetic o-aminobenzoyl peptide (Abz-based peptide substrate to release a free fluorophore). The released Abz can be easily quantified using a fluorescence microplate reader. In the presence of an ACE1 specific inhibitor, the enzyme loses its peptidase activity which results in decrease of fluorescence intensity. This assay kit is simple and can be used to identify and characterize ACE1 inhibitors in a high-throughput format.




 

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Description Size Supplier No. VWR Catalog Number Unit Price Quantity
Angiotensin I Converting Enzyme Activity (ACE) Assay Kit (Fluorometric) 100 Assays K228-100 75836-726 Each Retrieving

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

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