Simple method to isolate enriched or purified lysosomes from tissue and cultured cells.
- Simple & convenient protocol
- The kit provides unique formulations of ready-to-use buffers and reagents to isolate lysosomes with minimal sample preparation
- Isolation of high purity lysosomes from tissues and cultured cells
Lysosomes are membrane-bound cell organelles found in most animal cells (except red blood cells). Lysosomes are spherical vesicles containing hydrolytic enzymes capable of breaking down different kinds of biomolecules, including proteins, nucleic acids, carbohydrates, lipids, and cellular debris. They are known to contain over 50 different enzymes, which are active under acidic conditions. Thus, lysosomes act as the waste disposal system of the cells by digesting unwanted materials in the cytoplasm. BioVision’s Lysosome Isolation Kit provides a procedure for isolating enriched or purified lysosomal fraction from animal tissues and cultured cells by differential centrifugation followed by density gradient centrifugation.
