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8-Octanoyloxypyrene-1,3,6-trisulfonic acid trisodium salt

Supplier: MARKER GENE TECHNOLOGIES MS

M1296
Highly polar, highly water soluble lipase substrate is a derivative of HPTS (pyranine) that forms tight micelles in aqueous solutions.

  • Lipase subtrate

Alkyl esters of HPTS (sometimes called Cascade Blue) are well known for their substrate ability to measure esterase activity. Longer chain derivatives function as lipase substrates. Lipase activity can be measured by monitoring the sensitive fluorescence kinetics following the increase in emission of product HPTS (hydroxypyrene trisulfonic acid) as a function of time. The activity of a number of lipases have been measured using this substrate including wheat germ lipase, candida cylindracea lipase as well as carboxylic ester hydrolase and acylase 1. Because of its relatively low pKa value (7.3) under assay conditions, it is partially dissociated to form an anion that shows strong absorption maximizing at 460 nm and fluoresces with very high quantum yield and maximum intensity at 520 nm. Unfortunately, when excited between 440 and 480 nm, the fluorescence intensity is dependent on the pH of the solution, which requires careful pH control. However, when excited at 415 nm (the isosbestic point), no pH dependence of fluorescence intensity at 520 nm is noted.


Formula: C₂₄H₂₁Na₃O₁₁S₃
MW: 650.57 g/mol
CAS Number: 115787-84-3

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Size Supplier No. VWR Catalog Number Unit Price Quantity
25 mg M1296 101447-544 Each (25mg) Retrieving Restricted
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