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This reduced form of ethidine shows a blue fluorescence (absorption/emission: 355/420 nm) in cell cytoplasm until oxidization to form ethidium which becomes red fluorescent (absorption/emission: 518/605 nm) upon DNA intercalation.

  • Detects oxidative activities in viable cells
  • Exhibits increased fluorescence in various models of apoptosis

Dihydroethidium is a neutral probe and is able to penetrate the cell membrane of live cells, staining their cytoplasm blue as well as the chromatin/nucleus of living cells red. It typically exhibits a uniform labeling of cells within 30-40 minutes. Cell lysis of dihydroethidium-labeled cells can best be accomplished using Triton X-100 containing buffers. In addition, dihydroethidium has been incorporated into high-throughput assays to measure the effect of secondary agents or drugs on ROS activity in a live cell format.

Formula: C₂₁H₂₁N₃
MW: 315.42 g/mol
CAS Number: 104821-25-2



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10 mg M1241 101447-534 Each (10mg) Retrieving Restricted
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