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Ultrasensitive substrate for chemiluminescent measurement of β-galactosidase activity in homogeneous assays, or in cell lysate samples when the enzyme luciferase is added.

  • β-galactosidase substrate
  • Useful for coupled assays with luciferase

This analog of D-Luciferin contains a β-galactoside attached at the 6-O-position, and thus is not a substrate for the firefly luciferase enzyme until the galactose is removed e.g., by β-galactosidase activity. When combined with firefly luciferase in coupled assays, the combined double substrate (Luc-Gal) has proven to have detection limits for β-galactosidase activity as low as 3 x 10(-19) mol of enzyme per assay. It has also found utility in detection of engineered bacteria in vivo.

Formula: C₂₂H₂₄O₇
MW: 400,427 g/mol
CAS Number: 131474-38-9

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Size Supplier No. VWR Catalog Number Unit Price Quantity
5 mg M1087 101447-514 Each (5mg) Retrieving Restricted
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