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Highly fluorescent dye useful for oligonucleotide, antibody and protein labeling studies and for the preparation of peptide and esterase substrates.

  • Protein labeling reagent

Rhodol is a structural hybrid of fluorescein and rhodamine. It retains the pH-sensitive properties conferred by a phenolic hydroxyl group, but the amine substituent reduces the pKa values. Rhodol exhibits a high extinction coefficient (~70,000 cm-1M-1) and high quantum yield (~0.8-0.9) in aqueous solution, with absorption maxima at approximately 490 nm and emission maxima between 520 and 545 nm. As with fluorescein-based dyes, the fluorescence excitation profiles of rhodol-based dyes are pH dependent, which can be useful for ratiometric pH determinations intracellularly.

Formula: C₂₀H₁₃NO₄
MW: 331.327 g/mol
CAS Number: 3086-44-0



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Size Supplier No. VWR Catalog Number Unit Price Quantity
5 mg M0370 101176-268 Each (5mg) Retrieving Restricted
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