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Synonyms: 4-methylumbelliferyl-beta-D-glucuronide

Common blue fluorogenic GUS substrate for measuring cloned activity in plant lysate, homogenate, leaf disk or whole plant assays.

  • GUS (β-Glucuronidase) substrate

This fluorogenic substrate is especially useful for in vitro assays of β-glucuronidase activity arising from cloned GUS gene expression in plant lysate, homogenate, leaf disk or whole plant assays. β-Glucuronidase (GUS) encoded by the gusA gene from E.coli can cleave this substrate and generate the fluorogenic product 4-MU, that can be visualized or detected by irradiation with UV light. The fluorescence assay allows quantitation of GUS activity by means of a fluorimeter in protein extracts with excitation at 365nm (UV) and a peak emission at 455nm (blue). The fluorescence is optimal at pH values above 8-9.

Formula: C₁₆H₁₆O₉
MW: 352.3 g/mol
MDL Number: MFCD00150472
CAS Number: 6160-80-1



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Size Supplier No. VWR Catalog Number Unit Price Quantity
25 mg M0240 101176-214 Each (25mg) Retrieving Restricted
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