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N-(NBD-Aminohexanoyl) sphingosine


Fluorescent membrane probe. This C6-NBD-ceramide analog is used to detect, at the cellular level, a variety of lysosomal storage diseases as well as intracellular distribution and transport of the labeled sphingolipid molecules in living cells.

  • Membrane probe

Ceramides are the biological building blocks of more complex sphingolipids. Metabolism of ceramides typically occurs in Golgi and endoplasmic reticulum membranes. This fluorescent analog of sphingosine ceramide is incorporated into metabolic pathways and can be used as a vital stain for the Golgi apparatus, endoplasmic reticulum, and nuclear envelope of mammalian cells. In the presence of 10 microM monensin the visibility of the fluorescence is diminished on the plasma membrane, whereas the appearance of the Golgi apparatus is amplified. This probe has been known to produce the two fluorescent metabolites intracellularly, sphingomyelin and glucocerebroside intracellularly in the Golgi apparatus, which can then be transported to the cell surface. Monensin inhibits the ability to remove the fluorescent metabolites sphingomyelin and glucocerebroside from the cell surface by back exchange.

Formula: C₃₀H₄₉N₅O₆
MW: 575.751 g/mol
CAS Number: 86701-10-2



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Size Supplier No. VWR Catalog Number Unit Price Quantity
1 mg M0239 101176-212 Each (1mg) Retrieving Restricted
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