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Resorufin-β-D-galactopyranoside

Supplier: MARKER GENE TECHNOLOGIES MS

M0203
Red fluorogenic substrate for lacZ β-galactosidase activity, intracellularly, or in vitro.

  • beta-galactosidase substrate

This stable fluorogenic substrate for lacZ β-galactosidase activity, releases resorufin upon β-galactosidase enzyme cleavage. Unlike FDG that requires a two-step hydrolysis to generate maximum fluorescence, resorufin β-D-galactopyranoside requires only a single-step hydrolysis reaction to attain full fluorescence making it especially useful for sensitive enzyme measurements in applications such as ELISAs or high-content screening (HCS) systems. The relatively low pKa (~6.0) of resorufin (the enzymatic hydrolysis product of resorufin β-galactopyranoside) with Ex/Em=573/585 nm permits continuous measurement of enzymatic activity. Resorufin β-galactopyranoside has also been used to quantitate β-galactosidase activity in single yeast cells by flow cytometry and to detect immobilized -galactosidase activity. Marker Gene produces a high-purity, molecular biology grade of this substrate for your research needs.


Formula: C₁₈H₁₇NO₈
MW: 375.333 g/mol
CAS Number: 95079-19-9
Specification Test Results
Synonyms 4,7-Diphenyl-1,10-phenanthroline, Bphen
Form White Powder
Storage Store at Room Temperature (15-30 °C)

 

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Size Supplier No. VWR Catalog Number Unit Price Quantity
10 mg M0203 101176-204 Each (10mg) Retrieving Restricted
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