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N-Succinimidyl 6-Biotinamidohexanoate

Supplier: MARKER GENE TECHNOLOGIES MS
Synonyms: 6-Biotinamidohexanoic Acid N-Succinimidyl Ester, N-Biotinyl-6-aminohexanoic Acid Succinimidyl Ester, N-[5-(N-Succinimidyloxycarbonyl)pentyl]biotinamide

This reactive biotin derivative contains an aminohexanoyl spacer group (termed "X") between biotin and the binding site for proteins, nucleic acids, or other biological molecules which helps to reduce potential quenching effects of fluorophores attached to the analyte molecule, since biotin binds to "deep" binding sites in avidin or streptavidin.

  • Protein label
  • Spacer "X" reduces quenching effects

The extra linking arm of this biotin derivative makes it more accessible for binding, and improves a variety of assays employing biotin-avidin or biotin-streptavidin systems. This amine reactive (succinimidyl ester, SE) derivative can be readily coupled to primary or secondary amines of analyte compounds or proteins under mild conditions. Biotin is a commonly used hapten that can be detected by using its strong affinity for the proteins avidin (from egg white) or streptavidin. Many avidin or streptavidin conjugates are available that have been fluorescently labeled or are conjugated to an antibody or other detection reagent. This biotin labeling compound has been used in many such detection schemes for peptides, proteins, organelle components, monoclonal antibody analyses, oligonucleotide and microbead labelings

Formula: C₂₀H₃₀N₄O₆S
MW: 454,54 g/mol
MDL Number: MFCD00065502
CAS Number: 72040-63-2

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50 mg M0783 101176-324 Each (50mg) Retrieving Restricted
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