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Carboxyfluorescein Diacetate, Succinimidyl Ester

Supplier: Promocell, Inc.
Synonyms: 5(6)-CFDA N-succinmidyl ester, 5(6)-Carboxyfluorescein diacetate succinimidyl ester, Carboxyfluorescein Diacetate, Succinimidyl Ester

CFSE or 5(6)-CFDA, SE is a useful cell membrane-permeable fluorescent tracer that can passively diffuse into viable cells and readily accumulates there, where it covalently attaches to intracellular proteins.

Hydrolyzed CFSE emits fluorescence and covalently-attached fluorescein molecules
seldom leak from cells, resulting in long-term cell labeling. The reagent itself is colorless and
nonfluorescent, but fluoresces brightly green once it is hydrolyzed by intracellular esterases. The labeling has been shown to be stable for formaldehyde or glutaldehyde fixation conditions.

CFSE-labeled cells can be monitored over several weeks in vivo. Therefore, CFSE is utilized for viable cell detection as well as for the long-term observation of cell activities by fluorescent microscopy. The excitation and emission wavelengths of CFSE-labeled cells are 495 nm and 519 nm, respectively.

Formula: C₂₉H₁₉NO₁₁
MW: 557,467 g/mol
MDL Number: MFCD00037466
CAS Number: 150347-59-4
Specification Test Results
Excitation / Emission Maxima λex\ λem: 495/519 nm (of hydrolysis product at pH 7.0)
Molecular Weight 557 Da
Molecular Formula C29H19NO11
Purity >98% (as determined by TLC)
Appearance White Solid
Formulation / Solubility Soluble in DMSO or DMF
Storage Store desiccated at -20 °C
Stability Protect from Light, especially when in solution


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Size Supplier No. VWR Catalog Number Unit Price Quantity
25 mg PK-CA707-90041 10180-980 Each (25mg) Retrieving Restricted

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