Solid phase, sandwich ELISA for the determination of Inactive form of GLP-1 (9-36/37) in human, mouse and rat EDTA plasma, and in cell culture supernatant.
- For research use only, not for use in diagnostic procedures
- Quantitatively measure inactive forms of GLP-1
- Determine GLP-1 in cell culture supernatant
GLP-1 (Glucagon Like Peptide-1) is a transcriptional product of proglucagon gene. This is the same as glucagon and one of the gastrointestinal hormones which is secreted from L cells of the lower section of the small intestine. GLP-1 (7-36) amide and GLP-1 (7-37) are active forms and these peptides are dissolved in blood by DDP4 (dipeptidyl peptidase-4) into GLP-1 (9-36) amide and GLP-1 (9-37) and these peptides lose their activities in a short period. GLP-1 is known as incretin, the same as GIP (Glucose-dependent Insulinotropic Polypeptide). It promotes secretion of insulin from β cells of the isles of Langerhans in the pancreas as glucose dependently. GLP-1 also suppresses secretion of glucagon from α cell of pancreas and concentration of glucose in blood after meals. Drugs of type 2 diabetes have been developed by utilizing the functional mechanism of GLP-1 such as blood sugar control through the pancreas, delayed gastric emptying and appetite suppression which contributes weight loss as extra-pancreatic effects. This ELISA kit can quantitatively measure inactive forms of GLP-1 such as GLP-1 (9-36) amide and GLP-1 (9-37) specifically. It can also quantitatively measure total GLP-1 in EDTA-plasma which does not contain DPP4 inhibitor since active forms GLP-1 in blood are rapidly transformed to inactive forms in two to five minutes in a half-period.
