Human Hexokinase (HK) Inhibitor Screening Kit (Colorimetric), BioVision Inc.

Supplier: BioVision
AB211114-100TEST
75832-908EA 1118.48 USD
75832-908
Human Hexokinase (HK) Inhibitor Screening Kit (Colorimetric), BioVision Inc.
Assays Enzyme Assays
A simple and high-throughput adaptable assay kit used to colorimetrically (450 nm) screen, study and characterize potential inhibitors of human Hexokinase.

  • Simple and HTP compatible
  • High-throughput adaptable
  • Can be performed in less than 30 minutes

Hexokinases (HK, EC: 2.7.1.1) are found in many organisms including bacteria, plants and mammals and play an important role in glucose metabolism. The Hexokinase family phosphorylates glucose and generates glucose-6-phosphate for glycolysis. Four Hexokinase isoforms (HK-I, II, III and IV) are found in numerous species. HK-I, HK-II and HK-III have low affinity for glucose, one of their natural substrates, while HK-IV show high affinity for this monosaccharide. Recent studies have found increased hexokinase activity in various human metastatic tumors. Moreover, Hexokinase II (HKII) is the main isoform of the Hexokinases and is responsible for malignant phenotypes. HKII binds to the outer mitochondrial membrane via the Voltage-Dependent Anion Channel (VDAC), a Porin-like protein. HKII has become an attractive therapeutic target for its role in cancer metastasis. BioVision’s Human Hexokinase II (HKII) Inhibitor Screening kit uses the HKII’s ability to convert glucose into glucose-6-phosphate. Glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH, which reduces a probe that shows strong absorbance at 450 nm. In the presence of Bromopyruvic Acid, reactions are impeded, thus decreasing the rate and/or extent of generation of Hexokinase -dependent absorbance at OD 450 nm. Hexokinase Inhibitor Control is included to compare the efficacy of test inhibitors. This kit provides a sensitive, quick, and easy method for screening potential inhibitors of Hexokinase. The assay is high-throughput adaptable and can be performed in less than 30 minutes.
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