2-NBDG Glucose Uptake Assay Kit (Cell-Based), BioVision Inc.Supplier: BioVision
75832-872EA 813.3 USD75832-872
2-NBDG Glucose Uptake Assay Kit (Cell-Based), BioVision Inc.
Assays Cellular Assays
Non-radioactive method to detect and accurately measure glucose uptake via FACS and microscopy.
- Measurement of glucose uptake in response to insulin, growth factors, cytokines, mitogens and nutrients, etc.
- Dual-staining of glucose transporters and glucose uptake
- Analysis of glucose metabolism and cell signaling in various cell types
- Screening of anti-diabetic compounds
Glucose is a ubiquitous energy source in most organisms and plays a pivotal role in cellular metabolisms and homeostasis. Cancer cells exhibit increased glucose uptake to support their high proliferation rate. 2-NBDG (2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl) amino]-D-glucose) is a fluorescent deoxyglucose analog that can be taken up by cells through glucose transporters. However, 2-NBDG cannot be fully utilized in glycolysis because of its modification and thus accumulates inside the cells. Fluorescence generated by this fluorescent glucose analog is proportional to glucose uptake by the cells and can be used to measure glucose uptake using fluorescent microscopy and flow cytometry. To validate the assay, the kit includes phloretin, a natural phenol that inhibits glucose uptake. This easy to use non-radioactive kit allows imaging and accurate measurement of glucose uptake in cultured cells in response to insulin, growth factors etc.