Lipid Extraction Kit (Chloroform Free), BioVision Inc.

Supplier: BioVision
AB211044-50TEST
75833-280EA 637.8 USD
75833-280
Lipid Extraction Kit (Chloroform Free), BioVision Inc.
Protein Extraction Protein Extraction Kits
A chloroform free method of lipid isolation by organic extraction in a phase-free manner.

  • Phase free, chloroform free method of lipid isolation
  • Use with multiple downstream assays
  • Isolate up to 50 lipid preparations based on a 25 µl sample size

Lipids are a group of naturally occurring molecules that include monoglycerides, diglycerides, trigylcerides, fats, sterols and others. Lipids define and preserve cellular membrane integrity and play an important role in cellular processes such as membrane trafficking, signal transduction and energy storage. Dysregulation of lipid metabolism is linked to many diseases like cancer, diabetes and heart disease. Traditionally, the Folch method of organic extraction of lipids is preferred, in which lipids are extracted into a bottom organic phase. Isolating the lipids from this lower fraction is tricky and can in part cause isolation of other phases as well. This leads to low purity of lipid samples which might negatively affect or hinder downstream lipid analysis. In addition, this method uses chloroform as the organic phase solvent, which is toxic and long-term exposure to it is hazardous. BioVision’s Lipid Extraction Kit provides a chloroform free protocol of lipid isolation by organic extraction in a phase-free manner. The recovered organic phase can be dried and resuspended for further downstream lipid analysis. This protocol not only overcomes the difficulty of lower phase extraction and the dangers of using chloroform, but also provides for a very efficient and clean lipid extraction. Each kit provides sufficient reagents to isolate up to 50 lipid preparations based on a 25 µl sample size.

Caution: Store at RT
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