Industry's first kit to screen, study, characterize potential inhibitors of HRV 3C Protease.
- Screening, studying, characterizing inhibitors of HRV 3C Protease
- Simple and high-throughput adaptable assay kit
- Easy quantification
Human rhinovirus (HRV) infections are the most frequent causative agents of common cold and various other upper respiratory tract infections. Rhinoviruses are members of the picornavirus family, which have a positive-sense, single-stranded RNA genome that is translated into a single polyprotein precursor. In the case of HRVs, the viral polyprotein is mainly processed by the proteases (2A and 3C) to generate functional proteins and enzymes. BioVision’s HRV 3C Protease Inhibitor Screening Kit utilizes the ability of a 3C Protease (derived from a HRV rhinovirus-14, EC: 3.4.22.28) to cleave a chromogenic peptide substrate to release a chromophore (pNA) which can be easily quantified using a microplate reader. In the presence of a HRV 3C Protease-specific inhibitor, the cleavage of the substrate is reduced/abolished resulting in decrease or total loss of the absorbance. This simple and high-throughput adaptable assay kit can be used to screen, study, and characterize potential inhibitors of HRV 3C Protease.
