Nitric Oxide Synthase (NOS) Activity Assay Kit (Colorimetric), BioVision

Supplier: BioVision
75832-946EA 768.58 USD
Nitric Oxide Synthase (NOS) Activity Assay Kit (Colorimetric), BioVision
Assays Protein Assays
Ultrasensitive, Simple, HTP adaptable colorimetric assay kit for NOS activity measurement.

  • Detection of NOS activity
  • Simple, sensitive and high-throughput adaptable
  • Can detect as low as 5 µU of NOS activity

Nitric oxide synthases (EC (NOS) are a family of enzymes that catalyze the production of nitric oxide (NO) from L-arginine. Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. In presence of NADPH, FAD, FMN, (6R)-5,6,7,8-tetrahydrobiopterin, calmodulin and heme, NOS catalyzes a five-electron oxidation of the guanidino nitrogen of L-arginine with molecular oxygen to generate NO and L-citrulline. There are three isoforms of NOS: endothelial (eNOS), neuronal (nNOS), and inducible (iNOS). nNOS accounts for the production of NO in central nervous system, where NO participates in cell communication and information storage. eNOS produces NO in blood vessels and is involved in regulation of vascular function. In contrast to other isoforms, iNOS is expressed de novo under oxidative stress conditions and produces large amounts of NO as a part of body’s defense mechanism. BioVision’s Nitric Oxide Synthase Activity Assay Kit provides an accurate and convenient method to assay NOS activity in a variety of samples. In this assay, nitric oxide generated by NOS undergoes a series of reactions and reacts with Griess Reagent 1 and 2 to generate a colored product with a strong absorbance at 540 nm. The assay is simple, sensitive and high-throughput adaptable and can detect as low as 5 µU of NOS activity.
Order Now

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR