For ultra-sensitive and quantitative measurement of low-level β-galactosidase expression.
- Transfect cells with a plasmid expressing LacZ gene
- Very stable, resistant to proteolytic degradation and easy to assay
Levels of active β-galactosidase expression can be quickly measured by its catalytic hydrolysis of Chlorophenol red-β-D-galactopyranoside (CPRG) substrate to a dark red product. The assay kit provides all the required reagents and offers a rapid, simple and sensitive method to quantify the enzyme expression in transfected cells. The high sensitivity improves the measurement of β-gal activity when the reporter gene expression is low.
Delivery information: Kit includes ?-gal Enzyme Standard (40 units) 100 µL, 5X Lysis Buffer 55 mL, Standard Dilution Buffer 55 mL, Substrate Buffer 55 mL, Stop Buffer 55 mL and 10X CPRG Substrate Stock 5 x 1 mL.