The Dicer siRNA Generation Kit allows easy generation of a large number of small interfering RNAs (siRNAs) from full-length target genes. This kit mimics the natural RNA interference process by using recombinant human dicer enzyme, a double-stranded RNA-specific endonuclease, to cleave in vitro transcribed dsRNA templates into a pool of 22 bp siRNAs.
- A mixture of siRNAs has a higher chance of success than a single siRNA design
- More regions of the genes can be screened for silencing
- Ideal for use with GeneSilencer® siRNA Transfection Reagent
siRNAs are 21 to 23 nucleotide RNA molecules that can cause targeted gene silencing in mammalian cells through a process known as RNA interference
The TurboScript™ T7 Transcription Kit utilizes a novel technology to allow rapid synthesis of 10 to 50 times the amount of RNA produced by conventional in vitro transcription reactions. Kit is ideal for preparing double-stranded RNA for use with the Dicer siRNA Construction Kit.
The RNA Purification Column 1 is ideal for purifying siRNA generated with the Dicer™ siRNA construction kit or dsRNA generated with the TurboScript™ T7 transcription kit. RNA Purification Column 2 is ideal for purifying d-siRNA generated with the Dicer™ siRNA Construction Kit. Column 1 remove salts and unincorporated nucleotides from siRNA while Column 2 remove undigested dsRNA from siRNA. Each Column is sufficient for purifying 0.5 µg - 1.0 µg of siRNA or dsRNA.
In nature, siRNAs are generated by ribonuclease III cleavage of longer double stranded RNAs (dsRNAs). When dsRNAs are transfected directly into mammalian cells, they activate the interferon system and provoke non-specific gene suppression and cytotoxic response. siRNAs have proven to be effective at specifically silencing gene expression without causing any interferon response. The Dicer siRNA Generation Kit mimics the natural RNA interference process by using recombinant human dicer enzyme, a double-stranded RNA-specific endonuclease, to cleave in vitro transcribed dsRNA templates into a pool of 22 bp siRNAs. Kit contains everything that is required for preparing double stranded RNA, RNA cleavage, siRNA cleanup and transfection.
Ordering information: Recombinant Dicer Enzyme can be ordered separately.