The SoluLyse-M™ Mammalian Protein Extraction Reagent is a novel nonionic detergent designed for efficient whole-cell protein extraction from cultured mammalian cells
- High yields of non-denatured proteins
- Optimized protocols for both adherent and suspension cells
- Simple and rapid protocols
- Compatible with downstream applications
SoluLyse-M™ Reagent extracts cytoplasmic and nuclear protein from cultured cell lines using a proprietary non-ionic detergent in 25mM Tris, pH 7.4 and 250mM Sucrose. Reagent is compatible with many different applications, such as reporter assays, immunoassays and protein purification. The reagent is easily dialyzable and compatible with protein assays such as Western blotting, Coomassie Blue, Bradford and the BCA™ Protein Assays.
It gently and rapidly dissolves cell membranes at low concentrations without denaturing proteins.