TurboCells® BL21 Chemically Competent E. coli, Genlantis

Supplier: Genlantis
C302020 C303020
10497-456EA 578.11 USD
10497-456 10497-454
TurboCells® BL21 Chemically Competent E. coli, Genlantis
Competent Cells
TurboCells® BL21(DE3) and TurboCells® BL21(DE3)pLysS chemically competent E.coli incorporate a unique 3-minute transformation protocol with efficient transgene expression from T7 promoter-based vectors.

  • Fast and easy protocol
  • Ideal for high-throughput transformations and routine cloning experiments
  • Allow efficient transformation without the need for a lengthy transformation reaction

These strains contain the λDE3 lysogen, which expresses T7 RNA polymerase under the control of a lacUV5 promoter

When using the 3-minute transformation protocol, transformation efficiencies of up to 5 x 105 cfu/µg of supercoiled DNA can be achieved, which is more than sufficient for most protein expression experiments. When using the traditional 1.5 to 2 hour transformation protocol with the TurboCells BL21(DE3) and TurboCells BL21(DE3)pLysS Competent E. coli, greater transformation efficiencies can be achieved.

Upon addition of IPTG, the expression of the T7 RNA polymerase is induced, which allows for high level protein expression from T7 promoter-based expression vectors (e.g., pET). Additionally, BL21(DE3)pLysS contains the pLysS plasmid which produces T7 lysozyme, an inhibitor of T7 RNA polymerase, to reduce basal level expression of proteins that are toxic in E.coli.

Delivery information: Supplied in single-use 50 µL aliquots containing TurboCells® chemically competent E.coli, TurboCells® transformation buffer (5 mL) and pUC19 positive control plasmid (20 µL, 10pg/µL).
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