ICEme Mutation Enrichment Kits, Transgenomic

Supplier: Transgenomic
716172 716175 716112 716145 716106 716152 716102 716142 716122 716132
75815-616KT 11913.77 USD
75815-616 75815-580 75815-592 75815-608 75815-612 75815-568 75815-576 75815-588 75815-572 75815-584
ICEme Mutation Enrichment Kits, Transgenomic
Assays qPCR Assays
Transgenomic’s ICEme Kit is an in vitro assay that uses multiplexed PCR (MX PCR) followed by multiplexed ICE COLD-PCR (MX-ICP) for amplification and enrichment of mutations in KRAS Exons 2, 3 and 4, NRAS Exons 2, 3 and 4, BRAF Exons 11 and 15, PIK3CA Exons 9 and 20, EGFR Exons 12, 18, 19, 20 and 21.

  • Non-Allele Specific
  • Enriches ANY and ALL variants
  • Suppresses wild-type while amplifying mutations
  • Improves mutation detection sensitivity for any DNA sample
  • Compatible with any sequencing platform
  • LOD of mutations down to as low as 0.01%
  • Can be incorporated easily into any laboratory work flow

Multiplexed ICE COLD-PCR (Improved and Complete Enrichment CO-amplification at Lower Denaturing temperature) technology preferentially enriches mutant DNA sequences in an excess of wild-type DNA through selective amplification. The result is up to a 500-fold increase in sensitivity in identifying mutations with the most precise sequence alteration detection rates available – down to 0.01%. The ICEme kits are compatible with DNA isolated from any sample type (tissue, blood, plasma, saliva, etc.) and are platform-independent, enabling analysis on ALL sequencing platforms (Sanger, NGS and ddPCR).

Ordering information: Kits with 8 or 24 reactions are available. All kits include MX PCR Primer Mix, MX-ICP Primers, RS-oligos, MX-ICP Positive Control, Polymerases, PCR Buffers and dNTPs.
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