Anti-Actin Mouse Monoclonal Antibody [clone: C4]

Supplier: MP Biomedicals
08691001 08691002
10222-120EA 633.92 USD
10222-120 10221-880
Anti-Actin Mouse Monoclonal Antibody [clone: C4]
Antibodies
Mouse anti-actin, monoclonal (clone C4), is a mouse IgG1 (gamma 1 heavy/kappa light chain) antibody.

Chicken gizzard actin was used as the immunogen. Immunoblots demonstrate specificity directed towards all actins ranging from yeast, to flies, to mouse, to man. These are skeletal alpha-actin, cardiac alpha-actin, smooth muscle gamma actin, cytoplasmic beta-actin, cytoplasmic gamma actin as well as Dictyostelium discoideum and Physarum olycephalum actins. The epitope for the antibody lies between residues 18 to 40, a highly conserved region of the molecule. This antibody can also be used for cells cultivated on slides, for frozen tissue sections and as a loading control for western blots.

Indirect Immunofluorescence: 1:500, Immunoblots: 1:500 for DAB/peroxidase detection, actin band migrates at 42 kDa on SDS-page. Immunohistochemistry: 1:500.

Actin monoclonal antibody was used for western blot analysis of Cos-7 cell lysates to ensure equal protein loading. Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody. Also it's used in Indirect ELISA, Immunofluorescence, Immunoblots and Immunohistochemistry.
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