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AlbumiNZ™ Bovine Serum Albumin (BSA), Low Endotoxin, Stem Cell Grade, MP Biomedicals

Supplier: MP BIOMEDICALS (FKA ICN BIOMED
Bovine albumin is a single polypeptide chain consisting of approximately 583 to 595 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.

  • Mycoplasmas and viruses not detected

Albumins are a group of acidic proteins which occur in the body fluids and tissues of mammals and in some plant seeds. Serum and plasma albumin is carbohydrate-free and comprises 55-62% of the protein present. However, only about 40% of the total albumin in the body is in the circulating plasma at one time with the remainder being in extracellular spaces with which there is, in general, equilibration about every 24 hours.

It loses its helical structure rapidly in 2-4 M urea and 4 M guanidinium chloride. The unfolding is reversible, even, to some extent, after concomitant reduction of all disulfide bonds . Reduced albumin can reform into structures capable of binding antibodies and other ligands . Formation of SS bonds is rapid but the return of native structure is slow and some wrong disulfide pairings may persist . After oxidation of the cystine bridges, however, native structure is irreversibly lost.

It binds water, Ca2+, Na+, and K+. Due to a hydrophobic cleft, albumin binds fatty acids, bilirubin, hormones and drugs. The main biological function of albumin is to regulate the colloidal osmotic pressure of blood and to a lesser degree to provide cellular nutrition. Human and bovine albumins contain 16% nitrogen and are often used as standards in protein calibration studies . Albumin is used to solubilize lipids, stabilize protein solutions, and is also used as a blocking agent in Western blots or ELISA applications. Globulin free albumins are suitable for use in applications where no other proteins should be present such as electrophoresis.



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Size Packaging Supplier No. VWR Catalog Number Unit Price Quantity
10 g Bottle 0219989610 IC219989610 Each Retrieving
25 g Bottle 0219989625 IC219989625 Each Retrieving
100 g Bottle 0219989680 IC219989680 Each Retrieving
500 g Bottle 0219989690 IC219989690 Each Retrieving
1 kg Bottle 0219989691 IC219989691 Each Retrieving
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SPECIFICATIONS

Protein/Peptide Name Albumin
Protein Synonyms Cohn Fraction V, Bovine Serum Albumin, Fraction V, BSA Fraction V, BSA, Cohn Fraction V, Bovine Serum Albumin BSA, Bovine Serum Albumin, Cohn Fraction V, Fraction V Bovine Serum Albumin, Fraction V, BSA, Cohn Fraction V Fraction V, Bovine Serum Albumin, BSA, Cohn Fraction V Bovine Serum Albumin, Fraction V, Cohn Fraction V, BSA
Species Bovine
Source Serum
CAS No. 9048-46-8
Grade Stem Cell Grade
Purity ≥97% Total Protein
Endotoxin Content <1.0 EU/mg
Molecular Weight 66 kDa
Concentration ≥95%
Storage Conditions +4 °C
Color Off-white to Light Yellowish to Brown
Solubility Dissolves completely in 20 min at 20-25 °C (20%)
pH 6.5 - 7.5 (4% aq soln)
Physical Form Powder
Bioburden <100 cfu/g
Moisture Content ≥5%
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