96 wells (1 kit) - ELISA is a competitive enzyme immunoassay for the quantitative measurement of testosterone.
The BioVendor Mouse/Rat Testosterone ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. An unknown amount of testosterone present in the sample and a defined amount of testosterone conjugated to horseradish peroxidase compete for the binding sites of testosterone antiserum coated to the wells of a microplate. After one-hour incubation on a shaker the microplate is washed four times. After addition of the substrate solution the concentration of testosterone is inversely proportional to the optical density measured.
Ordering information: For research use only. Not for use in diagnostic or therapeutic procedures.
Caution: This is intended for research use only.