The Colorimetric Cell Viability Kits I allows for easy and reliable colorimetric determination of viable cell numbers with excellent sensitivity and linearity.
- Ideally suited for multiplexing measurements
- Does not show any cytotoxic effects
- Utilizes the tetrazolium salt WST-8
The Colorimetric Cell Viability Kits I allows for easy and reliable colorimetric determination of viable cell numbers with excellent sensitivity and linearity. The kits utilize the tetrazolium salt WST-8 that is reduced to water-soluble, orange formazan dyes by dehydrogenases present in viable cells. The absorbance of the formazan dye is proportional to the number of metabolically active cells and can be measured directly from a 96-well plate without additional processing. The ready-to-use WST-8 assay solution does not show any cytotoxic effects even with quite sensitive cell types after many hours of incubation. Thus, the assay is ideally suited for multiplexing measurements.