PARP-10. PARP-10 mono(ADP-ribosyl)ates (MARylates) NEMO (NF-kappaB essential modulator), which results in reduced NEMO polyubiquitylation and thus decreased NF-kappaB signaling. Overexpression of PARP-10 was shown to lead to apoptosis. PARP-10 knockdown leads to increased cell survival. Additional substrates of PARP-10, such as GSK-3beta were identified and PARP-10-mediated mono(ADP-ribosyl)ation (MARylation) was shown to be involved in regulating multiple processes, including Wnt signaling.
Immunocytochemistry:(1:50-1:200). Immunoprecipitation:(1:10). Western Blot:(1:50). Optimal conditions must be determined individually for each application.
Type: Primary
Antigen: PARP10
Clonality: Monoclonal
Clone: 5H11
Conjugation: Unconjugated
Epitope:
Host: Rat
Isotype: IgG1
Reactivity: Human