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CytoSelect™ BrdU Cell Proliferation ELISA Kit, Cell Biolabs

Supplier: Cell Biolabs
CBA-251
The CytoSelect™ BrdU Cell Proliferation ELISA Kit detects BrdU incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody

When cells are incubated in media containing BrdU, the pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells. Once the labeling media is removed, the cells are fixed and the DNA is denatured in one step with a fix/denature solution (denaturation of the DNA is necessary to improve the accessibility of the incorporated BrdU for detection). Then an anti-BrdU mouse monoclonal antibody is added followed by an HRP conjugated secondary antibody to detect the incorporated BrdU. The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells and can be directly correlated to cell proliferation.




 

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Description Size Method Supplier No. VWR Catalog Number Unit Price Quantity
BrdU Cell Proliferation ELISA Kit 96 Assays Colorimetric CBA-251 102963-848 Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

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SPECIFICATIONS

Storage Temperature 4°C and –20°C
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