Human Recombinant Ubiquitin Activ Enzyme E1

Supplier: BioVision
AB285724-50UG
10008-526EA 1041.88 USD
10008-526
Human Recombinant Ubiquitin Activ Enzyme E1
Proteins and Peptides
>95% Pure Human recombinant Ubiquitin Activating Enzyme E1

Typical enzyme concentration of 5 nM – 100 nM is used for in vitro conjugation, depending on conditions.

UBE1, the ubiquitin activating enzyme, is the first enzyme utilized in the pathway to conjugate ubiquitin onto target substrates through an ubiquitin-protein isopeptide bond. The C-terminal glycine of an ubiquitin (G76) is initially adenylated in an ATP dependent manner activating it so it can be transferred to the active site cysteine of UBE1 forming an ubiquitin-UBE1 thiolester. With the help of the other enzymes in the ubiquitin conjugation pathway (E2/E3 enzymes) the ubiquitin is transferred to a specific lysine on a target substrate.
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