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Human recombinant Ubiquitin Activating Enzyme E1, BioVision

Supplier: BioVision
>95% Pure Human recombinant Ubiquitin Activating Enzyme E1

Typical enzyme concentration of 5 nM – 100 nM is used for in vitro conjugation, depending on conditions.

UBE1, the ubiquitin activating enzyme, is the first enzyme utilized in the pathway to conjugate ubiquitin onto target substrates through an ubiquitin-protein isopeptide bond. The C-terminal glycine of an ubiquitin (G76) is initially adenylated in an ATP dependent manner activating it so it can be transferred to the active site cysteine of UBE1 forming an ubiquitin-UBE1 thiolester. With the help of the other enzymes in the ubiquitin conjugation pathway (E2/E3 enzymes) the ubiquitin is transferred to a specific lysine on a target substrate.



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Size Supplier No. VWR Catalog Number Unit Price Quantity
50 μg 6429-50 10008-526 Each (50µG) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

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To order chemicals, medical devices, or other restricted products please provide identification that includes your business name and shipping address via email or fax 484.881.5997 referencing your VWR account number . Acceptable forms of identification are:

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Protein/Peptide Name Ubiquitin Activ Enzyme E1
Protein Synonyms UBA1, GXP1, SMAX2, POC20, UBE1, A1ST, AMCX1, A1S9T
Protein/Peptide Type Recombinant
Species Human
Purity ≥95%
Molecular Weight 118 kDa
Formulation In 50 mM Tris (pH 8.0), 100 mM NaCl and 10% glycerol.
Protein Type Recombinant
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