Generate high efficiency next generation sequencing (NGS) DNA fragment libraries for Illumina sequencers starting with only 75 ng of sheared DNA input using this fast, PCR-free DNA library preparation kit.
- High-efficiency next generation sequencing (NGS)
- Optimized to ensure peak performance
- Prevents the introduction of PCR bias in the sequencing results
- Compatible with a larger number of starting samples
- Produces more sequenceable DNA fragments
- Better coverage and depth from single or multiplexed libraries
Libraries generated with this kit are suitable for de novo whole genome sequencing, resequencing, mutation/SNP detection and more. Each step of the NxSeq AmpFREE Low DNA Library Kit protocol is optimized to ensure peak performance on Illumina sequencers and provide the most sequencing data possible from each library. By avoiding the use of a PCR amplification step, this kits prevents the introduction of PCR bias in the sequencing results. The low input amount, 75 ng of sheared DNA, makes this kit compatible with a larger number of starting samples. These same samples would not be suitable for other library construction kits because those kits require more starting DNA.
The high efficiency of this kit produces more sequenceable DNA fragments, better coverage and depth from single or multiplexed libraries. The protocol is a quick, 2 hour and 10 minutes which saves valuable time and gets the library samples on the sequencer sooner. Each NxSeq Adaptor is identical except for a unique index/barcode sequence which is read by the Illumina sequencer to identify the adaptor used to make each library. Libraries made with different indexed adaptors can be pooled (multiplexed) in a single Illumina sequencer run, and then differentiated bioinformatically.
The NxSeq AmpFREE Kits and NxSeq Adaptors are only compatible with Illumina sequencing instruments (e.g. NextSeq, MiSeq and the HiSeq 2500, 3000, 4000, X Ten, and X Five).
For research use only. Not for human or diagnostic use.
Ordering information: Each NxSeq AmpFREE Library Kit contains Enzyme Mix (EM), 2X Buffer (2XB), Ligase (LIG) and Elution Buffer (EB) with enough reagents for the construction of 12 or 48 DNA fragment libraries. Each box of NxSeq Adaptors contains (12) different indexed Illumina-compatible adaptors with enough of each adapter for 4 library constructions. Box 1 contains adaptors 1-12 and Box 2 contains adaptors 13-24.