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IL-2 Receptor α, Human Recombinant, BioVision

Supplier: BioVision
7100-50 7100-10
>98% Pure active Human Recombinant IL-2 Receptor α.

Determined by its ability to increase the proliferation effect of IL-2 in mouse CTLL-2 cells. In the presence of 1 ng/ml of recombinant IL-2, the expected ED50 for this effect is between 0.5-1.5 μg/ml.

The IL-2 receptor system consists of three non-covalently linked subunits termed IL-2Rα, IL-2Rβ, and IL-2Rγ. The IL-2Rα is a type I transmembrane protein consisting of a 219 amino acid extracellular domain, a 19 amino acid transmembrane domain and a 13 amino acid intracellular domain, which is not involved in the transduction of IL-2 signals. Proteolytic processing of IL-2Rα releases the entire extracellular domain of IL-2Rα thereby generating a 219 amino acid soluble protein called soluble IL-2Rα (sIL-2Rα). The homodimeric form binds IL-2 (KD=10mM) and facilitates IL-2 signaling. The secreted sIL-2Rα is expressed on leukemia cells, lymphoma cells, newly activated T and B cells, as well as on approximately 10% of NK cells. Recombinant human sIL-2Rα is a 24.8 kDa protein containing 219 amino acid residues consisting of only the extracellular domain of IL-2Rα. Due to glycosylation, IL-2Rα has an approximate molecular weight of 31 kDa based on SDSPAGE gel and Mass Spectrometry.




 

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Size Supplier No. VWR Catalog Number Unit Price Quantity
10 μg 7100-10 10009-060 Each (10µG) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

50 μg 7100-50 10009-062 Each (50µG) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

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SPECIFICATIONS

Protein/Peptide Name Il 2 Receptor ?
Protein Synonyms soluble IL-2 receptor
Protein/Peptide Type Recombinant
Species Human
Source BTI-TN-5B1-4 Hi-5 Insect cells
Purity ≥98%
Endotoxin Content < 0.1 ng/μg of protein (<1EU/μg).
Molecular Weight 24.8 kDa
Formulation Sterile filtered through a 0.2 micron filter. Lyophilized from 1x PBS
Reconstitution Instructions Reconstitute in 1x PBS pH 7.2 to a concentration of 0.1-1.0 mg/ml. Do not vortex Reconstitute in 1x PBS pH 7.2 to a concentration of 0.1-1.0 mg/ml. Do not vortex.
Protein Type Recombinant
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