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Caspase-9 Fluorometric Assay Kit, BioVision

Supplier: BioVision

Caspase-9 Fluorometric Assay Kit, BioVision

Caspase-9 Fluorometric Assay Kit, BioVision

AB65607-100

10013-546EA 504.79 USD

10013-546

Caspase-9 Fluorometric Assay Kit, BioVision

Assays Protein Assays

Convenient, Highly-Sensitive Assay

fluorecence (400 nm excitation filter and 505 nm emission filter)
Mammalian
Convenient sizes (25, 100, 200, 400 assays)
Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-9 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on detection of cleavage of substrate LEHD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEHD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by caspase-9 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader.

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