Caspase-8 Colorimetric Assay Kit, BioVision

Supplier: BioVision
AB39700-100
10013-514EA 518.06 USD
10013-514
Caspase-8 Colorimetric Assay Kit, BioVision
Assays Protein Assays
Convenient, Highly-Sensitive Assay

  • Absorbance (400 or 405 nm)
  • Mammalian
  • Convenient sizes (25, 100, 200, 400 assays)
  • Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The FLICE/Caspase-8 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate IETD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
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