- Detection method- Absorbance (450 nm)
- Species reactivity- Mammalian
- Applications- This assay detects human Resistin in the range of 0.125–8 ng/ml resistin/ml. The lowest level of resistin that can be detected by this assay is 100 pg/ml.
Obesity is a well-known risk factor of type 2 diabetes mellitus and is strongly associated with insulin resistance. Resistin (also called FIZZ3/ADSF) is an adipocyte-derived peptide first identified during a search for targets of thiazolidinediones. Serum concentrations of resistin are markedly increased in obese mice and are decreased by treatment with thiazolidinediones. It was also found that administration of an anti-resistin antibody increases insulin-stimulated glucose uptake in obese mice and that treatment of normal mice with recombinant resistin impairs insulin action. Thus, resistin might link obesity with insulin resistance and diabetes in mice models. However, subsequent studies in rodent models have produced disparate findings on the role of resistin in obesity and insulin resistance. In humans, while the expression of resistin in human adipocytes is very low compared with that seen in rodents and does not differ between normal, insulin-resistant or type 2 diabetic individuals, a more recent study using a large size of case suggests that the plasma resistin levels are increased in type 2 diabetes. Therefore determination of the plasma resistin levels may be important for understanding onsets of metabolic diseases such as type 2 diabetes or obesity. This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human resistin in biological fluids. A monoclonal antibody specific for resistin has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, resistin is recognized by the addition of a biotinylated polyclonal antibody specific for resistin (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of resistin in the samples. The assay range is 0.125–8 ng/ml resistin/ml. The lowest level of resistin that can be detected by this assay is 100 pg/ml.