HMG-CoA Reductase Activity/Inhibitor Screening Kit (Colorimetric), BioVision

Supplier: BioVision
AB204701-100TEST
10013-762EA 1165.7 USD
10013-762
HMG-CoA Reductase Activity/Inhibitor Screening Kit (Colorimetric), BioVision
Assays Protein Assays
Simple assay to measure activity of purified enzyme and screening inhibitors/activators of HMGR

  • Detection method- Absorbance (OD 340 nm)
  • Applications- Measurement of HMG-CoA reductase activity
  • - Screening/studying/characterizing HMG-CoA reductase inhibitors/activators

HMG-CoA reductase (3-hydroxy-3-methyl-glutaryl-CoA reductase or HMGR) (EC 1.1.1.34) is the rate-controlling enzyme of the mevalonate pathway, the metabolic pathway that produces cholesterol from acetyl-CoA. In an NADPH-dependent reaction, HMG-CoA reductase reduces HMG-CoA to generate mevalonate and CoA. The enzyme is target of a group of cholesterol-lowering drugs known as statins. Inhibition of HMG-CoA reductase induces expression of LDL receptors in the liver, which lowers plasma concentration of cholesterol. BioVision’s HMG-CoA Reductase Activity Assay Kit measures utilization of NADPH, which can be measured by the decrease of absorbance (OD 340 nm). The kit is suitable for measuring activity of purified enzyme and screening inhibitors/activators of HMG-CoA reductase. The limit of detection is below 0.05 mU.
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