Beta-Secretase Activity Assay Kit, BioVision

Supplier: BioVision
AB65357-100
10013-686EA 688.56 USD
10013-686
Beta-Secretase Activity Assay Kit, BioVision
Assays Protein Assays
Highly Sensitive Assay

  • Detection method- Fluorescence (Ex/Em 335-355/ 495-510 nm)
  • Species reactivity- Mammalian
  • Applications- Convenient fluorescence method for detecting β-secretase activity in biological and purified samples.

β-Secretase has been implicated to be an excellent target for anti-amyloid therapy for the treatment of Alzheimer’s disease. The β-Secretase activity Assay Kit provides a convenient fluorescence method for detecting β-secretase activity in biological and purified samples. The assay utilizes a secretase-specific peptide conjugated to two reporter molecules EDANS and DABCYL. In the uncleaved form, the fluorescent emissions from EDANS are quenched by the physical proximity of the DABCYL moiety. Cleavage of the peptide by secretase physically separates EDANS and DABCYL allowing for the release of a fluorescent signal. The level of secretase enzymatic activity in samples is proportional to the level of fluorescence intensity.
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