Human Recombinant UBCH5B (from E. coli), HIS Tag

Supplier: BioVision
AB111451-10UG AB111451-100UG
10007-210EA 406.02 USD
10007-210 10007-212
Human Recombinant UBCH5B (from E. coli), HIS Tag
Proteins and Peptides
>95% Pure active Recombinant human UbcH5b.

UbcH5b is highly homologous to the S. cerevisiae UbC4/5 family of E2 enzymes, demonstrated to be essential for degradation of regulatory and abnormal proteins. UbcH5b, together with UbcH5a and UbcH5c, are the most active class of E2 enzymes in cell extracts and are associated with regulation of a number of transcription factors including p53. Useful for in vitro ubiquitinylation reactions.The His-tagged version of this enzyme is not susceptible to self-ubiquitinylation, which can occur with GST-tagged versions. Typical enzyme concentration to support in vitro conjugation is 100 nM to 1 µM depending upon the conditions.
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