Caspselect Caspase-3 Immunoassay Kit, BioVision

Supplier: BioVision
AB234045-100TEST
10013-626EA 1348.31 USD
10013-626
Caspselect Caspase-3 Immunoassay Kit, BioVision
Assays Protein Assays
Specifically detects Caspase-3

  • Fluorescence (Ex/Em 370-425/ 490-525 nm)
  • Mammalian
  • 100 assays
  • Effective immunosorbent enzyme assay for specific, quantitative detection of caspase-3 activity in microtiter plates

Activation of caspase-3 plays a key role in initiation of cellular events during apoptosis. The CaspSELECT™ Caspase-3 Immunoassay Kit provides an effective immunosorbent enzyme assay for specific, quantitative detection of caspase-3 activity in microtiter plates. The assay utilizes caspase-3 polyclonal antibody to capture activated caspase-3 from cell lysates. Caspase substrate DEVD-AFC is then added and is cleaved proportionally to the amount of activated caspase-3 in the cell lysate. The cleavage generates free AFC which can be analyzed fluorometrically (Ex./Em. = 400 nm/505 nm) using a fluorescence plate reader. The assay ensures absolute specific detection of caspase-3. Other known caspases and non-specific proteases are not detected.
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