Caspase-12 Fluorometric Assay Kit, BioVision

Supplier: BioVision
AB65664-100
10013-296EA 581.65 USD
10013-296
Caspase-12 Fluorometric Assay Kit, BioVision
Assays Protein Assays
Convenient, Highly-Sensitive Assay

  • Fluorescence (Ex/Em 400/505)
  • Mammalian
  • Convenient sizes (25, 100 assays)
  • Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis

Caspase family of proteases are the central mediators of apoptosis in mammalian cells. The Caspase-12 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence ATAD. The assay is based on detection of cleavage of substrate ATAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). ATAD-AFC emits blue light (lmax = 400 nm); upon cleavage of the substrate by caspase-12 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader.
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