Keep my session open?
Ending In 
Your shopping session has expired.
Your session has expired. For your security, we have logged you out.
Would you like to log in again?

Gibson Assembly® HiFi 1 Step Master Mix, Synthetic Genomics Inc.

Supplier: SGI - DNA Synthetic Genomics
GA1100-50MM GA1100-10MM
Generate seamless plasmid DNA or BAC clones without restriction digestion

The Gibson Assembly process begins by designing dsDNA fragments with 20 – 40bp overlapping ends. For the Gibson Assembly HiFi 1-Step reaction, these DNA fragments are combined with a 2X Gibson Assembly® HiFi 1-Step Master Mix and incubated. This during this time, complementary ends are generated, annealed, and sealed. This this one-step, isothermal process can be performed in less than 60 minutes and results in a transfection or transformation ready, double stranded, fully ligated DNA construct.

The GA 1-Step Master Mix (2X) contains a proprietary mixture of enzymes and reagents optimized to facilitate one-step assembly of double stranded DNA fragments. This mastermix includes a proof-reading polymerase that mediates junction repair resulting assembled constructs with low rates of junction errors and high sequence fidelity. This product does not include the GA Positive Control found in the Gibson Assembly® HiFi 1-Step Kit. If you are new to Gibson Assembly or require a validated positive control for you experiments, you may want to consider catalog numbers GA1100-S, GA1100-10, or GA1100-50.

The Gibson Assembly method allows the insertion of one or more linear double stranded DNA fragments into a virtually any vector without the need to rely on compatible restriction sites. Gibson Assembly is significantly faster than traditional restriction enzyme digest-based cloning and proven for the cloning of both small and large double stranded DNA fragments. Using the Gibson Assembly® HiFi 1-Step Master Mix, the simultaneous cloning of one to five inserts with sizes ranging from 500 bp to 32kb can be accomplished in a single tube, in a single step, using a robust isothermal reaction that yields cloning efficiencies of greater than 90%.

Ordering information: Master mixes supporting 10, or 50 reactions are available. Master mixes are provided as a single vial of GA 1-Step Master Mix (2X) along with a detailed instruction manual. Note that this product does not include the GA Positive Control found in the Gibson Assembly® HiFi 1-Step Kit. If you are new to Gibson Assembly or require a validated positive control for you experiments, you may want to consider catalog numbers GA1100-S, GA1100-10, or GA1100-50.



 

ORDER

Expand All / Collapse All
Description No. of Reactions Supplier No. VWR Catalog Number Unit Price Quantity
Gibson Assembly® HiFi 1 Step Master Mix 50 GA1100-50MM 10820-616 Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

Gibson Assembly® HiFi 1 Step Master Mix 10 GA1100-10MM 10820-612 Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

restricted In order to process your orders without delay, we request that you provide the required business documentation to purchase this product.

To order chemicals, medical devices, or other restricted products please provide identification that includes your business name and shipping address via email CMD_NA@vwr.com or fax 484.881.5997 referencing your VWR account number . Acceptable forms of identification are:

  • State issued document with your organization's Federal Tax ID Number
  • State issued document with your organization's Resale Tax ID Number
  • City or County issued Business License
  • State Department of Health Services License
  • Any other ID issued by the State that includes the business name and address

* Please note if your account is within the State of California two of these pieces of identification will be required.
VWR will not lift restrictions for residential shipping addresses.

Generate Barcodes Barcode Label Format: 
Generate PDF Catalog Page

Enter Details

Select the article for which you are looking for Certificate

Indicate the Lot Number. This field is mandatory.

Enter Certificate Details

Enter the Lot number:

Choose from recent batch/lot numbers:

Or
Review & Compare Alternatives
We found alternative products that can save you up to  per item-unit.  To compare product details, select up to 3 alternatives below and click Compare Selected. To add items to your basket, enter a quantity and click Add to Basket.

Original Product:

Description Catalog Number Availability Unit Your Price Price Per Qty
 

A new look and a renewed commitment

Built on the features & resources you know, optimized with innovation from Avantor®. Our new brand symbolizes agility and our ability to adapt to customer needs.

Learn More Start Shopping