Pierce™ S-Nitrosylation Western Blot Kit, Thermo Scientific

Supplier: Thermo Fisher Scientific
Pierce
90105 90106 90106 90105
PI90105EA 614.15 USD
PI90105 PI90106 CAPI90106 CAPI90105
Pierce™ S-Nitrosylation Western Blot Kit, Thermo Scientific
Blotting Kits
Thermo Scientific Pierce S-Nitrosylation Western Blot Kit enables sensitive detection of protein S-nitrosocysteine post-translational modifications in a complete, easy-to-use kit.

  • Optimized labeling reactions switch S-nitrosocysteine post translational modifications with iodoTMT Reagent for easy detection by Western blot using anti-TMT antibody
  • Uses a non-biological iodoTMT Reagent for labeling instead of HPDP-biotin, resulting in less background during Western blot detection
  • Labeling method is compatible with alternative methods of analysis, including enrichment via anti-TMT resin and multiplex quantitation by mass spectrometry
  • All needed reagents are included one kit

The Pierce™ S-Nitrosylation Western Blot Kit contains a cell lysis/reaction buffer, a sulfhydryl-reactive blocking agent, a reducing agent, a labeling agent and a detection monoclonal antibody. Each kit has enough reagents to label 40 samples of 100 µg each. Unmodified cysteines are first blocked using a sulfhydryl-reactive compound (MMTS). S-nitrosylated cysteines are then selectively reduced with ascorbate in HENS Buffer for specific labeling with iodoTMTzero™ Reagents, which irreversibly bind to the cysteine thiol that was S-nitrosylated. Detection of the TMT™ reagent-modified proteins is facilitated using an anti-TMT antibody.

Delivery information: The kit includes HENS Buffer (100 mL), methyl methanethiosulfonat (MMTS, 200 mg), sodium ascorbate (20 mg), iodoTMTzero™ label reagent (2 x 0.2 mg) and anti-TMT™ antibody (100 µL).

Caution: For Research Use Only. Not for use in diagnostic procedures.
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