Horseradish Peroxidase

Supplier: Pierce Chemical
31491 31490 31491 31490
PI31490EA 125.68 USD
CAPI31491 CAPI31490 PI31490 PI31491
Horseradish Peroxidase
Thermo Scientific Pierce Horseradish Peroxidase (HRP) is purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.

  • Purified form—lyophilized salt-free powder; ready to dissolve and use
  • High specific activity—typically greater than 300 units/mg (lot-specific value reported)
  • Compared to AP—HRP is smaller (40kDa) than alkaline phosphatase (AP; 140kDa) and has higher specific enzyme activity than both AP and beta-galactosidase (b-Gal)
  • Many options—numerous substrate solutions and assay techniques are available for HRP

This purified horseradish peroxidase (HRP) is supplied lyophilized as a salt-free powder for reconstitution and use in protein research methods

One unit catalyzes the production of 1 mg of purpurogallin from pyrogallol in 20 seconds at 20˚C and pH 6.0.

The main application for HRP in molecular biology and protein research is as a reporter system for immunoassays and other probe-based assay techniques such as ELISA, Western blotting, EMSA and Southern blotting. The enzyme is usually conjugated to specific secondary antibodies or streptavidin, and its activity is detected with a color-forming (or light-generating) substrate.

Ordering information: Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, but do not store above ?20?C.

Caution: For Research Use Only. Not for use in diagnostic procedures.
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