These Iodination Beads are polystyrene beads that are coated with an oxidizing reagent, which provides efficient activation of 125I for protein or peptide iodination procedures.
- Derivatised, uniform, non porous polystyrene beads
- Iodine-125 incorporation as high as 99%; labeled protein recovery >90%
- Able to function over a broad pH and temperature range
- Iodinates in the presence of azides, detergents, urea and high salt
- Allows efficient iodination of cell membrane surface proteins
- Fast and easy to use – iodinations complete in 2 to 15 minutes
- Reaction stopped by simply removing beads from reaction mixture with tweezers or pasteur pipette; no reducing agent necessary to terminate reaction
The coated iodination beads provide for convenient and efficient iodine-labeling without the hassles and damaging oxidative effects normally associated with chloramine T and other solution-based methods
Iodination involves the introduction of radioactive iodine into certain amino acids (usually tyrosines) in proteins and peptides. Iodination takes place at the positions ortho to the hydroxyl group on tyrosine; mono- or di-substitution can occur. When iodinatable sites such as tyrosines are absent or of limited accessibility in a protein, iodinatable phenolic sites can be introduced by using the Bolton-Hunter Reagents (SHPP and Sulfo-SHPP). Certain crosslinkers also contain iodinatable tyrosyl groups in their spacer arms.
One or two beads can be added per milliliter of protein solution and then easily removed following the labeling reaction, thereby completely removing the oxidizing reagent from the protein.
Caution: For Research Use Only. Not for use in diagnostic procedures. Upon receipt store product desiccated at 4 °C. Avoid exposing beads to moisture or reducing agents. Product is shipped at ambient temperature.
