3-[(3-Cholamidopropyl)dimethylammonio]-1-propane sulfate (CHAPS) zwitterionic detergent, Pierce™Supplier: Thermo Scientific
Thermo Scientific CHAPS is a zwitterionic detergent that is especially well suited for protecting the native state of proteins.
- Versatile zwitterionic detergent
- Provides mild but effective lysis of cultured mammalian cells
- Nondenaturing and generally does not inactivate protein functions
- Can be removed from solution by dialysis
- Commonly used for isoelectric focusing (IEF) and 2D electrophoresis
CHAPS is sulfobetaine derivative of cholic acid
CHAPS has been successfully used to solubilize intrinsic membrane proteins and receptors and maintain the functional capability of the protein of interest. CHAPS has also been used in combination with nonionic detergents such as NP-40 for nondenaturing gel electrophoresis applications. CHAPS has largely replaced NP-40 in isoelectric focusing (IEF) applications, where it prevents streaking in certain pH ranges. The neutral charge of CHAPS and its disaggregating properties are believed to be responsible for the improvement.
Properties of CHAPS Detergent:
Chemical Name: 3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate
Molecular Weight: 614.88g
Detergent Class: Zwitterionic
Aggregation Number: 10
Micelle Molecular Weight: 6149g
Critical Micelle Concentration (CMC): 8 to 10 mM (0.4920 to 0.6150%, w/v)
Cloud Point: ≥100˚C
Specifications for CHAPS:
Visual: White free-flowing powder, free of foreign matter.
Solubility: A 10% (aq) solution is clear, colorless.
Identity: The I.R. scan must show only peaks characteristic for the structure and functional groups of the compound being tested.
Purity: (TLC) One major spot. A small, secondary spot is acceptable.
Caution: For Research Use Only. Not for use in diagnostic procedures.
MW: 614.89 g/mol
Melting Pt: 116 °C
Storage Temperature: Refrigerator
CAS Number: 75621-03-3
Merck Index: 12,02085